{Author}:陈有钟 黄 捷 余 辉 程元荣 徐国兴 谢茂松 徐 蕾

　　{Year}:2014

　　{Title}:雷帕霉素微乳滴眼剂的制备及其在大鼠角膜移植排斥反应的应用

　　{Journal}:《中国抗生素杂志》

　　{Volume}:39

　　{Issue}:9

　　{Pages}: 679-682

　　{ISBN/ISSN}:1001-8689

　　{Keywords}:雷帕霉素 微乳 滴眼剂 角膜移植 排斥反应

　　{Abstract}:目的制备雷帕霉素微乳滴眼剂,评价其抑制大鼠角膜移植排斥反应的疗效。方法采用微乳制备方法,研制雷帕霉素微乳滴眼剂;通过大鼠双眼滴加药液进行粘膜刺激性试验和对大鼠角膜移植排斥反应的作用,评价雷帕霉素微乳滴眼液治疗大鼠角膜移植的效果。结果雷帕霉素微乳滴眼剂为透明或半透明的淡蓝色均一溶液,其平均粒径129.5nm,对粘膜刺激性小;有效减少角膜移植排斥反应的发生,显着延长角膜植片的存活时间。结论雷帕霉素微乳滴眼剂能够用于治疗大鼠角膜移植手术后的排斥反应。

　　Objective To prepare rapamycin microemulsion eye drops and evaluate its inhibition on corneal graft rejection in rats.Methods Rapamycin microemulsion eye drops was prepared by microemulsion method.The inhibition effect of rapamycin microemulsion eye drops on corneal graft rejection was evaluated by rat mucous membrane irritation test and rat corneal allograft transplantation. Results The appearance of rapamycin microemulsion eye drops was transparent or translucent light blue uniform solution.